Journal: International journal of biological macromolecules

Article Title: Enhancing epithelial regeneration with gelatin methacryloyl hydrogel loaded with extracellular vesicles derived from adipose mesenchymal stem cells for decellularized tracheal patching.

doi: 10.1016/j.ijbiomac.2024.137927

Figure Lengend Snippet: Fig. 2. (a,b) Immunohistochemical (IHC)expression of the Major Histocompatibility Complex (MHC) and (c)scanning electron microscope (SEM)image of decel- lularized trachea.

Article Snippet: Immunogenic components were evaluated by immunohistochemical staining of frozen sections (IHC-F) using antibodies against major histocompatibility complex (MHC) antigens, specifically MHC-I (dilution 1:200; MCA810GA, Bio-Rad, USA) and MHC-II (dilution 1:200; MCA811GA, Bio-Rad, USA).

Techniques: Immunohistochemical staining, Expressing, Immunopeptidomics, Microscopy

Journal:

Article Title: Granulocyte-Macrophage Colony-Stimulating Factor Priming plus Papillomavirus E6 DNA Vaccination: Effects on Papilloma Formation and Regression in the Cottontail Rabbit Papillomavirus-Rabbit Model

doi:

Figure Lengend Snippet: GM-CSF DNA inoculation induces GM-CSF mRNA expression and MHC class II protein expression. (A to C) The detection of GM-CSF mRNA expression (black grains) by in situ hybridization is shown for rabbit skin 1 (A), 2 (B), and 5 (C) days after GM-CSF DNA inoculation. Normal control skin showed no signal (data not shown). (D to F) The detection of cell surface expression of rabbit MHC class II protein (pink stain) by immunohistochemical analysis is shown for normal control skin (D) and rabbit skin 3 days following GM-CSF DNA inoculation (E and F). The tissue sections in panels E and F were photographed at magnifications of ×8.4 and ×84, respectively.

Article Snippet: Immunohistochemical analysis was performed on periodate-lysine-paraformaldehyde-fixed frozen sections with alkaline phosphatase-conjugated mouse anti-rabbit MHC class II monoclonal antibody clone 45-3 (Accurate Chemical and Scientific Corp., Waterbury, N.Y.) and HistoMark Red (Kirkegaard and Perry Laboratories, Gaithersburg, Md.).

Techniques: Expressing, In Situ Hybridization, Staining, Immunohistochemical staining

Journal: PLoS ONE

Article Title: Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells

doi: 10.1371/journal.pone.0111390

Figure Lengend Snippet: A. After osteogenic differentiation of rabbit MSCs and Alizarin red staining, calcium deposition is observed (arrows; original magnification, 100×; scale bar, 10 µm). B. After chondrogenic differentiation of rabbit MSCs and Alcian blue staining, sulfated cartilage GAGs are observed (arrows; original magnification, 100×; scale bar, 100 µm). C. After adipogenic differentiation of rabbit MSCs and Oil Red O staining, accumulation of intracellular neutral lipid vacuoles is observed (arrows; original magnification, 100×; scale bar, 10 µm).

Article Snippet: Secondary antibodies were used following staining of rabbit MSCs with mouse anti-rabbit CD14, CD44, CD45, MHC I, MHC II (all from Antigenix America, Huntington Station, NY), and α-SMA (AbD Serotec Ltd., Oxford, UK) monoclonal antibodies; with rat anti-rabbit CD34, desmin (all from GeneTex), and CD90 (Thermo Fisher Scientific) monoclonal antibodies; and with rabbit anti-rabbit CD106 and CD166 (both from Bioss Inc.) polyclonal antibodies.

Techniques: Staining

Journal: PLoS ONE

Article Title: Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells

doi: 10.1371/journal.pone.0111390

Figure Lengend Snippet: Mean percentages of rabbit MSCs that were labeled with each of the 25 markers, as determined by flow cytometry.

Article Snippet: Secondary antibodies were used following staining of rabbit MSCs with mouse anti-rabbit CD14, CD44, CD45, MHC I, MHC II (all from Antigenix America, Huntington Station, NY), and α-SMA (AbD Serotec Ltd., Oxford, UK) monoclonal antibodies; with rat anti-rabbit CD34, desmin (all from GeneTex), and CD90 (Thermo Fisher Scientific) monoclonal antibodies; and with rabbit anti-rabbit CD106 and CD166 (both from Bioss Inc.) polyclonal antibodies.

Techniques: Labeling, Cytometry

Journal: PLoS ONE

Article Title: Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells

doi: 10.1371/journal.pone.0111390

Figure Lengend Snippet: A. After osteogenic differentiation of human MSCs and Alizarin red staining, calcium deposition is observed (arrow, original magnification, 100×; scale bar, 10 µm). B. After chondrogenic differentiation of human MSCs and Alcian blue staining, sulfated cartilage GAGs are observed (arrows; original magnification, 100×; scale bar, 100 µm). C. After adipogenic differentiation of human MSCs and Oil Red O staining, accumulation of intracellular neutral lipid vacuoles is observed (arrows; original magnification, 100×; scale bar, 10 µm).

Article Snippet: Secondary antibodies were used following staining of rabbit MSCs with mouse anti-rabbit CD14, CD44, CD45, MHC I, MHC II (all from Antigenix America, Huntington Station, NY), and α-SMA (AbD Serotec Ltd., Oxford, UK) monoclonal antibodies; with rat anti-rabbit CD34, desmin (all from GeneTex), and CD90 (Thermo Fisher Scientific) monoclonal antibodies; and with rabbit anti-rabbit CD106 and CD166 (both from Bioss Inc.) polyclonal antibodies.

Techniques: Staining

Journal: PLoS ONE

Article Title: Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells

doi: 10.1371/journal.pone.0111390

Figure Lengend Snippet: Mean percentages of human MSCs that are labeled with each of the 25 markers, as determined by flow cytometry.

Article Snippet: Secondary antibodies were used following staining of rabbit MSCs with mouse anti-rabbit CD14, CD44, CD45, MHC I, MHC II (all from Antigenix America, Huntington Station, NY), and α-SMA (AbD Serotec Ltd., Oxford, UK) monoclonal antibodies; with rat anti-rabbit CD34, desmin (all from GeneTex), and CD90 (Thermo Fisher Scientific) monoclonal antibodies; and with rabbit anti-rabbit CD106 and CD166 (both from Bioss Inc.) polyclonal antibodies.

Techniques: Labeling, Cytometry

Journal: PLoS ONE

Article Title: Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells

doi: 10.1371/journal.pone.0111390

Figure Lengend Snippet: The RT-PCR products from rabbit and human MSCs were normalized to the expression of β-actin. The electrophoresis of the rabbit MSCs showed a normalized expression of CD44 mRNA of 0.8, and CD105 mRNA of 0.59. The electrophoresis of the human MSCs showed a normalized expression of CD44 mRNA of 0.71, and CD105 mRNA of 2.4. The results indicated that the ratio between rabbit and human CD44 expression was 1∶0.89, while the ratio between rabbit and human CD105 expression was 1∶4.08. These results indicated that rabbit and human MSCs had similar expression of CD44 at the mRNA level. However, human MSCs, compared with rabbit MSCs, had apparent more expression of CD105 at the mRNA level.

Article Snippet: Secondary antibodies were used following staining of rabbit MSCs with mouse anti-rabbit CD14, CD44, CD45, MHC I, MHC II (all from Antigenix America, Huntington Station, NY), and α-SMA (AbD Serotec Ltd., Oxford, UK) monoclonal antibodies; with rat anti-rabbit CD34, desmin (all from GeneTex), and CD90 (Thermo Fisher Scientific) monoclonal antibodies; and with rabbit anti-rabbit CD106 and CD166 (both from Bioss Inc.) polyclonal antibodies.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Electrophoresis

Journal: PLoS ONE

Article Title: Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells

doi: 10.1371/journal.pone.0111390

Figure Lengend Snippet: Comparison of data from the current study with data from four previous studies that examined the phenotypes of human and rabbit MSCs.

Article Snippet: Secondary antibodies were used following staining of rabbit MSCs with mouse anti-rabbit CD14, CD44, CD45, MHC I, MHC II (all from Antigenix America, Huntington Station, NY), and α-SMA (AbD Serotec Ltd., Oxford, UK) monoclonal antibodies; with rat anti-rabbit CD34, desmin (all from GeneTex), and CD90 (Thermo Fisher Scientific) monoclonal antibodies; and with rabbit anti-rabbit CD106 and CD166 (both from Bioss Inc.) polyclonal antibodies.

Techniques: Marker, Expressing

Journal: PLoS ONE

Article Title: Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells

doi: 10.1371/journal.pone.0111390

Figure Lengend Snippet: Mean percentages of human MSCs that were labeled with each of 11 markers at passage 3 and 6.

Article Snippet: Secondary antibodies were used following staining of rabbit MSCs with mouse anti-rabbit CD14, CD44, CD45, MHC I, MHC II (all from Antigenix America, Huntington Station, NY), and α-SMA (AbD Serotec Ltd., Oxford, UK) monoclonal antibodies; with rat anti-rabbit CD34, desmin (all from GeneTex), and CD90 (Thermo Fisher Scientific) monoclonal antibodies; and with rabbit anti-rabbit CD106 and CD166 (both from Bioss Inc.) polyclonal antibodies.

Techniques: Labeling